lectin (Vector Laboratories)
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Vector Laboratories
lectin
Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 255 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wga+fluorescein/Fluorescein+labeled+Wheat+Germ+Agglutinin+(WGA)/pm41934404-269-10-11
Average 95 stars, based on 255 article reviews
Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 255 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wga+fluorescein/Fluorescein+labeled+Wheat+Germ+Agglutinin+(WGA)/pm41934404-269-10-11
Average 95 stars, based on 255 article reviews
lectin - by Bioz Stars,
2026-09
95/100 stars
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Incubation:Article Title: fishROI: A specialized workflow for semi-automated muscle morphometry analysis in teleosts Article Snippet: For Tg( actc1b : GFP) zebrafish, vibratome sections were mounted directly into 1% low-melt agarose, followed by confocal imaging on Leica SP8 multiphoton microscope (multiphoton functionality disabled). .. Other zebrafish vibratome sections were incubated with 2% goat serum block (2% goat serum, 1% BSA, 1% DMSO in PBST) for 1 hour, followed by overnight incubation with Alexa Fluor 546 phalloidin (Invitrogen, A22283, 1:400 dilution) and Article Title: The role of GCNT1 mediated O -glycosylation in aggressive prostate cancer Article Snippet: Next, slides were washed with PBS and blocked with Carbo-FreeTM Blocking solution (Vector Laboratories, SP-5040) for 1 h at room temperature. .. Slides were incubated overnight at 4 °C with FITC-conjugated LEA (Lectin from Lycopersicon esculentum , Sigma, L0401), RCA 120 Fluorescein ( Ricinus Communis Agglutinin 120, Vector Laboratories, FLK-2100), Article Title: The role of GCNT1 mediated O-glycosylation in aggressive prostate cancer. Article Snippet: Next, slides were washed with PBS and blocked with Carbo-FreeTM Blocking solution (Vector Laboratories, SP-5040) for 1 h at room temperature. .. Slides were incubated overnight at 4 °C with FITC-conjugated LEA (Lectin from Lycopersicon esculentum, Sigma, L0401), RCA 120 Fluorescein (Ricinus Communis Agglutinin 120, Vector Laboratories, FLK-2100), Blocking Assay:Article Title: fishROI: A specialized workflow for semi-automated muscle morphometry analysis in teleosts Article Snippet: For Tg( actc1b : GFP) zebrafish, vibratome sections were mounted directly into 1% low-melt agarose, followed by confocal imaging on Leica SP8 multiphoton microscope (multiphoton functionality disabled). .. Other zebrafish vibratome sections were incubated with 2% goat serum block (2% goat serum, 1% BSA, 1% DMSO in PBST) for 1 hour, followed by overnight incubation with Alexa Fluor 546 phalloidin (Invitrogen, A22283, 1:400 dilution) and Whole Genome Amplification:Article Title: fishROI: A specialized workflow for semi-automated muscle morphometry analysis in teleosts Article Snippet: For Tg( actc1b : GFP) zebrafish, vibratome sections were mounted directly into 1% low-melt agarose, followed by confocal imaging on Leica SP8 multiphoton microscope (multiphoton functionality disabled). .. Other zebrafish vibratome sections were incubated with 2% goat serum block (2% goat serum, 1% BSA, 1% DMSO in PBST) for 1 hour, followed by overnight incubation with Alexa Fluor 546 phalloidin (Invitrogen, A22283, 1:400 dilution) and Article Title: Wheat Germ Agglutinin Conjugated Fluorescent pH Sensors for Visualizing Proton Fluxes Article Snippet: .. Article Title: Divergence in skeletal muscle growth by differential spatial hyperplastic patterning in teleost fishes Article Snippet: Next, sections were washed into PBST, then incubated in secondary antibodies and dyes diluted in corresponding blocking solutions for 4h (cryosections) or overnight at 4°C (for vibratome sections) respectively, then washed into PBST. .. The following dyes and secondary antibodies were used: DAPI (1:300), Phalloidin 555 (1:400, Invitrogen, A30106), Phalloidin 647 (1:400, Invitrogen, A22287), Article Title: Wheat germ agglutinin–conjugated fluorescent pH sensors for visualizing proton fluxes Article Snippet: After stirring for 4 h, the solvent was evaporated under reduced pressure, and pHRho was purified by HPLC using a C18 column (4.6 mm × 250 mm) eluting with solvent A (0.1% trifluoroacetic acid) and solvent B (acetonitrile); gradient 5–95% B over 30-min pHRho (1.0 mg, 1.4 μmol) was reacted with 14 μmol N,N,N′,N′-tetramethyl-O-(N-succinimidyl) uronium tetrafluoroborate in 200 μl anhydrous N,N-dimethylformamide at room temperature for 1 h. The above reaction mixture (100 μl) was added to 1 mg WGA (Vector Laboratories) for a 5:1 dye/WGA molar ratio in 1 ml of 0.1 M sodium bicarbonate (pH 8.3) containing 120 mg N-acetylglucosamine. .. The reaction mixture was stirred at room temperature for 2 h and was terminated by adding 6.25 μl of a hydroxylamine solution to a final concentration of 0.1 M. Commercially available Article Title: Intestinal goblet cells sample and deliver lumenal antigens by regulated endocytic uptake and transcytosis Article Snippet: Chemical compound, drug , UEA1 Fluorescein , Vector Laboratories , Cat#FL-1061–2 , 10 μg/ml. .. Chemical compound, drug , Article Title: The role of GCNT1 mediated O -glycosylation in aggressive prostate cancer Article Snippet: Next, slides were washed with PBS and blocked with Carbo-FreeTM Blocking solution (Vector Laboratories, SP-5040) for 1 h at room temperature. .. Slides were incubated overnight at 4 °C with FITC-conjugated LEA (Lectin from Lycopersicon esculentum , Sigma, L0401), RCA 120 Fluorescein ( Ricinus Communis Agglutinin 120, Vector Laboratories, FLK-2100), Article Title: The role of GCNT1 mediated O-glycosylation in aggressive prostate cancer. Article Snippet: Next, slides were washed with PBS and blocked with Carbo-FreeTM Blocking solution (Vector Laboratories, SP-5040) for 1 h at room temperature. .. Slides were incubated overnight at 4 °C with FITC-conjugated LEA (Lectin from Lycopersicon esculentum, Sigma, L0401), RCA 120 Fluorescein (Ricinus Communis Agglutinin 120, Vector Laboratories, FLK-2100), Article Title: Conserved oligomeric Golgi complex specifically regulates the maintenance of Golgi glycosylation machinery Article Snippet: Alternatively, a signal was detected using an enhanced chemiluminescent reagent for HRP from Pierce (Rockford, IL) and quantified using imagej software ( http://rsb.info.nih.gov/ij/ ). .. Lectins (with their concentrations) used were as follows: G. simplicifolia lectin-II (GS-II)-Alexa 594 (100 μg/mL, Invitrogen, Carlsbad, CA), Alexa 488-Peanut Agglutinin (PNA) (20 μg/mL, Invitrogen), PNA-rhodamine (20 μg/mL, Vector Laboratories, Burlingame, CA), Galanthus nivalus lectin (GNL)-Alexa 647 (20 μg/mL, Vector), L. tetragonolobus lectin (LTL)-Alexa 647 (20 μg/mL, Vector) and WGA-rhodamine and Imaging:Article Title: fishROI: A specialized workflow for semi-automated muscle morphometry analysis in teleosts Article Snippet: For Tg( actc1b : GFP) zebrafish, vibratome sections were mounted directly into 1% low-melt agarose, followed by confocal imaging on Leica SP8 multiphoton microscope (multiphoton functionality disabled). .. Other zebrafish vibratome sections were incubated with 2% goat serum block (2% goat serum, 1% BSA, 1% DMSO in PBST) for 1 hour, followed by overnight incubation with Alexa Fluor 546 phalloidin (Invitrogen, A22283, 1:400 dilution) and Microscopy:Article Title: fishROI: A specialized workflow for semi-automated muscle morphometry analysis in teleosts Article Snippet: For Tg( actc1b : GFP) zebrafish, vibratome sections were mounted directly into 1% low-melt agarose, followed by confocal imaging on Leica SP8 multiphoton microscope (multiphoton functionality disabled). .. Other zebrafish vibratome sections were incubated with 2% goat serum block (2% goat serum, 1% BSA, 1% DMSO in PBST) for 1 hour, followed by overnight incubation with Alexa Fluor 546 phalloidin (Invitrogen, A22283, 1:400 dilution) and Concentration Assay:Article Title: Wheat germ agglutinin–conjugated fluorescent pH sensors for visualizing proton fluxes Article Snippet: After stirring for 4 h, the solvent was evaporated under reduced pressure, and pHRho was purified by HPLC using a C18 column (4.6 mm × 250 mm) eluting with solvent A (0.1% trifluoroacetic acid) and solvent B (acetonitrile); gradient 5–95% B over 30-min pHRho (1.0 mg, 1.4 μmol) was reacted with 14 μmol N,N,N′,N′-tetramethyl-O-(N-succinimidyl) uronium tetrafluoroborate in 200 μl anhydrous N,N-dimethylformamide at room temperature for 1 h. The above reaction mixture (100 μl) was added to 1 mg WGA (Vector Laboratories) for a 5:1 dye/WGA molar ratio in 1 ml of 0.1 M sodium bicarbonate (pH 8.3) containing 120 mg N-acetylglucosamine. .. The reaction mixture was stirred at room temperature for 2 h and was terminated by adding 6.25 μl of a hydroxylamine solution to a final concentration of 0.1 M. Commercially available |